The CEM Discover 2.0 uses microwave technology to hydrolyze proteins and peptides in preparation for amino acid analysis, finishing as quickly in 15 minutes. direct application of microwave energy allows for rapid heating and maintenance of the set temperature for each sample; the temperature is measured by patented iWave temperature sensors which provide accurate temperature reading of the sample itself. In situ stirring ensures even heat distribution through the sample and post reaction quenching cools the sample in seconds so that it is ready for further processing or analysis.
Physical Requirements
ï‚· The System has an electrical requirement of 240 VAC, 50 Hz, 10 Amp ï‚· The System requires a minimum of 25 psi and maximum of 60 psi compressed air or compressed nitrogen. ï‚· The System requires a laptop provided by CEM to be located within ten feet of the System.Reagents Required
1. 6N HCl for acid hydrolysis 1:1 Concentrated HCl: DI water 2. Performic acid (preoxidation only) 9:1 formic acid: 30% hydrogen peroxide 3. 10 mL or 35-mL vial for Discover 2.0 with appropriate stir bar and cap