Advanced Continuous Excitation Chlorophyll Fluorimeter
The Handy PEA chlorophyll fluorimeter consists of a compact, light-weight control unit encapsulating sophisticated electronics providing the high time resolution essential in performing measurements of fast chlorophyll fluorescence induction kinetics.
Up to 1000 recordings of between 0.1 – 300 seconds may be saved in the memory of Handy PEA chlorophyll fluorimeter. Saved data may be viewed onscreen in numerical format with calculated parameters or transferred to the PEA Plus software where it may be viewed graphically or exported to external software packages for further statistical analysis.
- Upload user-defined, repeatable protocols for automatic field execution
- Interchangeable sensor unit cables with lengths of up to 20 metres
- Powerful Windows® data transfer & analysis software included
Handy PEA+ consists of a compact, light-weight control unit encapsulating sophisticated electronics. This provides the high-time resolution which is essential in performing measurements of fast chlorophyll fluorescence induction kinetics.
Simple to configure and operate, basic measurement functionality can be defined directly on the Handy PEA+. More complex experimental design may be acheived using the protocols feature which allows up to 5 user-defined protocols to be stored in memory for different field applications. Protocols are written using a custom Windows® software package, PEA+ (supplied). A tactile keypad allows selections and inputs to be made and a liquid crystal display module presents menu options and data.
The sensor unit consists of an array of 3 ultra-bright red LED’s which are optically filtered to a peak wavelength of 650 nm (which is readily absorbed by chlorophyll) at a maximum intensity of up to 3500 µmol m-2 s-1 at the sample surface. The LED’s are focused via lenses onto the leaf surface to provide uniform illumination over the area of leaf exposed by the leafclip (4mm dia). An optical feedback circuit monitors and corrects changes in the output intensity of the LED’s which can be caused by internal heat build-up within the LED’s themselves. The circuit also compensates for intensity changes caused by variation in ambient temperature.
The sensor unit is fitted with a high-performance pin photodiode. Optical design and filtering ensure that it responds maximally to the longer wavelength fluorescence signal and blocks the reflected shorter wavelength LED light used as the source of illumination. Variable rate analysis allows fluorescence signals to be sampled at different acquisition rates throughout the different phases of the induction kinetic. Initially, data is sampled at 10 µsecond intervals for the first 300 µseconds with subsequent induction phases analysed at lower acquisition intervals as the rate of kinetic activity reduces. Up to 1000 recordings of between 0.1 – 300 seconds may be saved in the onboard memory of the Handy PEA+. Calculated parameters may be viewed onscreen with more comprehensive data display acheived by transferring saved data via USB to a PC where the supplied PEA+ Windows software allows a variety of numerical and graphical presentation options.
Handy PEA+ systems are supplied with the following components
- Handy PEA+ control unit and sensor
- HPEA/LC x 2: (20 Leafclips)
- Mains powered battery charger
- Protective carry bag
- USB data transfer cable
- USB Drive containing PEA+ software and manuals
